The Sekelsky Lab

pP{Target70}

pP{Target70i} is a derivative of pP{Target70}. We expected the hsp70::white cDNA construct to produce dark red eyes in most inserts, but instead we get all colors, from light yellow to dark red. We therefore put an intron back into the gene in the hopes that this would increase expression, but his hasn't yet been tested.

pP{Target70} Sequence

CATGATGAAATAACATAAGGTGGTCCCGTCGGCAAGAGACATCCACTTAACGTATGCTT GCAATAAGTGCGAGTGAAAGGAATAGTATTCTGAGTGTCGTATTGAGTCTGAGTGAGAC AGCGATATGATTGTTGATTAACCCTTAGCATGTCCGTGGGGTTTGAATTAACTCATAAT ATTAATTAGACGAAATTATTTTTAAAGTTTTATTTTTAATAATTTGCGAGTACGCAAAG ctagagttgttggttggcacaccacaaatatactgttgccgagcacaattccgctcgac GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCggatccAGATCTGCTAGCGGCCGCA TGCCGCGGACGCGTAGGCCTGCAGGAATTCGATTCGACCTCGAGGGGGGGCCCGGTACC ACCAAACTGTCTCACGACGTTTTGcaattctagtatgtatgtaagttaataaaacccat ttttgcggaaagtagataaaaaaaacattttttttttttactgcactggatccttgccc cgttattctctattcgttttgtgactctccctctctgtactattgctctctcactctgt cgcacagtaaacggcactctattctcgttgcttcgagagagcgcgcctcgaatgttcgc gaaaagagcgccggagtataaatagaggcgcttcgtcgacggagcgtcaattcaattca aacaagCAAAGTGAACACATCGCGAAGCAAGCTGAGCAAACAAACAAGCGCAGCGAACA AGCTAAACAATCTGCAATAAAGTGCAAGTTAAAGTGAATCAATTAAAAGTAACCAACAA CCAAGTAATTAAACTAAAAACTGCAACTACTGAAATCAACCAAGAAGTCATTATTGAAG ACAAGAAGAGAACTCTgaataggtcgatagcgtcaattcggcacgaggtttcgtgacga agctccaagcggtttacgccatcaattaaacacaaagtgctgtgccaaaactcctctcg cttcttatttttgtttgttttttgagtgattgggtgggtgattggttttgggtgggtaa gcaggggaaagtgtgaaaaatcccggcaATGGGCCAAGAGGATCAGGAGCTATTAATTC GCGGAGGCAGCAAACACCCATCTGCCGAGCATCTGAACAATTTGATATTTGAGATCCCC TATCATTGCAGGGTGACAGCGGACGCTTCGCAGAGCTGCATTAACCAGGGCTTCGGGCA GGCCAAAAACTACGGCACGCTCCTGCCACCCAGTCCGCCGGAGGACTCCGGTTCAGGGA GCGGCCAACTAGCCGAGAACCTCACCTATGCCTGGCACAATATGGACATCTTTGGGGCG GTCAATCAGCCGGGCTCCGGATGGCGGCAGCTGGTCAACCGGACACGCGGACTATTCTG CAACGAGCGACACATACCGGCGCCCAGGAAACATTTGCTCAAGAACGTTTGCGGCGTGG CCTATCCGGGCGAACTTTTGGCCGTGATGGGCAGTTCCGGTGCCGGAAAGACGACCCTG CTGAATGCCCTTGCCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCG ACTGCTCAATGGCCAACCTGTGGACGCCAAGGAGATGCAGGCCAGGTGCGCCTATGTCC AGCAGGATGACCTCTTTATCGGCTCCCTAACGGCCAGGGAACACCTGATTTTCCAAGCC ATGGTGCGGATGCCACGACATCTGACCTATCGGCAGCGAGTGGCCCGCGTGGATCAGGT GATCCAGGAGCTTTCGCTCAGCAAATGTCAGCACACGATCATCGGTGTGCCCGGCAGGG TGAAAGGTCTGTCCGGCGGAGAAAGGAAGCGTCTGGCATTCGCCTCCGAGGCACTAACC GATCCGCCGCTTCTGATCTGCGATGAGCCCACCTCCGGACTGGACTCATTTACCGCCCA CAGCGTCGTCCAGGTGCTGAAGAAGCTGTCGCAGAAGGGCAAGACCGTCATCCTGACCA TTCATCAGCCGTCTTCCGAGCTGTTTGAGCTCTTTGACAAGATCCTTCTGATGGCCGAG GGCAGGGTAGCTTTCTTGGGCACTCCCAGCGAAGCCGTCGACTTCTTTTCCTA
gtgagttcgatgtgtttattaagggtatctagcattacattacatctcaactcctatccag
CGTGGGTGCCCAGTGTCCTACCAACTACAATCCGGCGGACTTTTACGTACAGGTGTTGGCCGTTG TGCCCGGACGGGAGATCGAGTCCCGTGATCGGATCGCCAAGATATGGCGACAATTTTGC CATTAGCAAAGTAGCCCGGGATATGGAGCAGTTGTTGGCCACCAAAAATCTGGAGAAGC CACTGGAGCAGCCGGAGAATGGGTACACCTACAAGGCCACCTGGTTCATGCAGTTCCGG GCGGTCCTGTGGCGATCCTGGCTGTCGGTGCTCAAGGAACCACTCCTCGTAAAAGTGCG ACTTATTCAGACAACGAAGATAAATAAATTTCATTTAACTCGCGAATATTAATGAGATG CGAGTAACATTTTAATTTGCAGATGGTTGCCATCTTGATTGGCCTCATCTTTTTGGGCC AACAACTCACGCAAGTGGGTGTGATGAATATCAACGGAGCCATCTTCCTCTTCCTGACC AACATGACCTTTCAAAACGTCTTTGCCACGATAAATGTGTTCACCTCAGAGCTGCCAGT TTTTATGAGGGAGGCCCGAAGTCGACTTTATCGCTGTGACACATACTTTCTGGGCAAAA CGATTGCCGAATTGCCGCTTTTTCTCACAGTGCCACTGGTCTTCACGGCGATTGCCTAT CCGATGATCGGACTGCGGGCCGGAGTGCTGCACTTCTTCAACTGCCTGGCGCTGGTCAC TCTGGTGGCCAATGTGTCAACGTCCTTCGGATATCTAATATCCTGCGCCAGCTCCTCGA CCTCGATGGCGCTGTCTGTGGGTCCGCCGGTTATCATACCATTCCTGCTCTTTGGCGGC TTCTTCTTGAACTCGGGCTCGGTGCCAGTATACCTCAAATGGTTGTCGTACCTCTCATG GTTCCGTTACGCCAACGAGGGTCTGCTGATTAACCAATGGGCGGACGTGGAGCCGGGCG AAATTAGCTGCACATCGTCGAACACCACGTGCCCCAGTTCGGGCAAGGTCATCCTGGAG ACGCTTAACTTCTCCGCCGCCGATCTGCCGCTGGACTACGTGGGTCTGGCCATTCTCAT CGTGAGCTTCCGGGTGCTCGCATATCTGGCTCTAAGACTTCGGGCCCGACGCAAGGAGT AGccgacatatatccgaaataactgcttgttttttttttttaccattattaccatcgtg tttactgtttattgccccctcaaaaagctaatgtaattatatttgtgccaataaaaaca agatatgacctatagaatacaaaaaaaaaaaaaaaaaaaaaaaaaaaaactcgatcgag tcgacGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCggatccaaacctagtaatta attcggctgctgctctAAACGACGCATTTCGTACTCCAAAGTACGAATTTTTTCCCTCA AGCTCTTATTTTCATTAAACAATGAACAGGACCTAACGCACAGTCACGTTATTGTTTAC ATAAATGATTTTTTTTACTATTCAAACTTACTCTGTTTGTGTACTCCCACTGGTATAGC CTTCTTTTATCTTTTCTGGTTCAGGCTCTATCACTTTACTAGGTACGGCATCTGCGTTG AGTCGCCTCCTTTTAAATGTCTGACCTTTTGCAGGTGCAGCCTTCCACTGCGAATCATT AAAGTGGGTATCACAAATTTGGGAGTTTTCACCAAGGCTGCACCCAAGGCTCTGCTCCC ACAATTTTCTCTTAATAGCACACTTCGGCACGTGAATTAATTTTACTCCAGTCACAGCT TTGCAGCAAAATTTGCAATATTTCATTTTTTTTTATTCCACGTAAGGGTTAATGTTTTC AAAAAAAAATTCGTCCGCACACAACCTTTCCTCTCAACAAGCAAACGTGCACTGAATTT AAGTGTATACTTCGGTAAGCTTCGGCTTTCGACGGGACCACCTTATGTTATTTCATCAT Gggccagacccacgtagtccagcggcagatcggcggcggagaagttaagcgtctccagg atgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaatttcgcccgg ctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgaga ggtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaaggcgttt ttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg gcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcc actggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtg gtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagc cagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggt agcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaga agatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaag ggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaa tgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatg cttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgct gcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcc agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgtt gttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcgg ttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactc atggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttc tgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagtt gctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtg ctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgag atccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttca ccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagg gcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaa taggggttccgcgcacatttccccgaaaagtgccacctgcgaagtcttagagccagata
tgcgagcacccggaagctcacgatgagaatggccagac

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